FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43471521
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG48450.029.12279945
CGTATGC56100.025.33599546
TATGCCG58450.024.14028448
CTCGTAT60900.023.05582844
ACACGTC78250.019.04633913
ACGTCCG55500.018.58603532
GATCGGA69950.018.3575041
AGTCACG65800.017.35426528
TCTCGTA83200.017.12508843
ACGTCTG102950.014.84502615
CACGTCT103800.014.62399114
GTCACGT81600.014.16309729
ATCTCGT104100.013.42178842
ATGCCGT108250.013.19398649
TTACGCG25700.013.15517254
CACACGT121350.012.67964712
CGCACAT77800.012.50499737
CGTCCGC82750.012.34050133
GTCCGCA81150.012.28636534
TCACGTC86600.012.07065530
Produced by FastQC (version 0.11.5)