FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43471521
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG25650.014.58927454
TACGCGA30150.012.63953955
GCGGTAT60700.011.287531
ACGCGAC37450.010.45064456
CGACCTA22300.010.13678554
GTATCGA41350.010.0924714
CGTATCG38700.09.5258233
GCGGAAT47500.09.5185561
CGAATCG39300.09.47498352
GTTACGC40350.09.42455853
CGAAGAT129350.09.330061
TTACACG52200.09.26438632
TCGACCG41350.09.25612331
AATCGAA70550.09.0203085
ATCGAAC56300.09.0160086
GCGCGAT35850.08.9252421
CCCGTAT44850.08.917781
CCGGAAT93350.08.9044061
ATCGAAG90250.08.8603416
CCGGGAT90300.08.8585261
Produced by FastQC (version 0.11.5)