FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26611654
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA318460.11966937492874363TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG184450.032.18133545
GATCGGA161150.032.148321
AGATCGG111300.031.6959381
TATGCCG190750.031.29885148
CGTATGC190950.031.06784246
CTCGTAT187850.030.29752544
ACACGTC204650.030.16772713
ATCGGAA174450.029.5049612
AATCTCG192850.028.88712941
ACGTCTG214550.028.77569615
TCTCGTA194350.028.6818943
CACGTCT222250.027.96465714
ATCTCGT204200.027.04544642
ATGCCGT220550.026.9292549
CACACGT234350.026.3280412
TCGGAAG204950.025.4684013
GCACACG245450.025.43198811
CGTCTGA240350.024.6839816
GCCGTCT242450.024.45418451
GTATGCC253400.023.81885147
Produced by FastQC (version 0.11.5)