FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42821701
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA82800.043.59420450
GCGTCGT89050.040.63941612
GCCGTAT91300.039.5067248
CGCCGTA94250.038.41776747
GATCGGA103550.037.1786082
AGCGTCG110600.033.15767711
AGAGCGT117150.031.6563939
CCGTATC116500.030.92772349
AGATCGG127900.029.6628251
GTAGATC124900.029.08451332
TGGTCGC123750.028.23152243
CGTGTAG129550.028.16842816
CGTCGTG134400.027.62005613
GTCGTGT131700.027.47963114
TCGTGTA131950.027.3494815
ATCGGAA139550.027.2609843
TCGCCGT135000.026.43925946
GGTCGCC134650.026.40579844
GTATCAT140850.026.26229551
AAGAGCG148650.025.4757188
Produced by FastQC (version 0.11.5)