FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24323610
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG19950.021.9597843
CGTATGC22550.019.7337844
TATCGCG19500.019.4596522
TATGCCG23700.018.77627446
GATCGGA29100.015.9186991
ACACGTC30600.015.67275513
AGTCACG30000.015.18208728
CGCGTAA14550.014.9683291
CGTATCG20400.013.1892463
CTCGTAT33500.012.76862142
ACGGCTA40800.011.84010632
CGGTATA16550.011.6738812
TTACGCG15450.011.61042954
CGCGCGT21850.011.5496071
ATATCGC34450.011.51531421
ATCGCGG35100.011.49959523
GCGGTAT41800.011.2475611
CGGGGTT64900.011.2391521
GTATCGA21750.011.1015274
GCGGGTT25050.011.0402271
Produced by FastQC (version 0.11.5)