FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21128499
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG80400.027.88015745
TATGCCG83500.026.84508748
CGTATGC84850.026.41796946
GTTCCGT85950.025.723735
CTCGTAT86650.025.7124144
ACACGTC94550.024.50286913
TATCTCG87550.024.2806941
CGTATCT88550.024.19884539
GATCGGA96650.024.037471
AGTTCCG99350.023.6257734
TCCGTAT92600.023.47113237
CCGTATC91400.023.36974138
AGATCGG70000.023.0518951
ACGTCTG101050.023.02760915
TTCCGTA99600.022.19778836
TCTCGTA99150.022.12721443
CACGTCT105900.021.97325114
ATCGGAA107600.021.618322
ATGCCGT103900.021.50872649
GCCGTCT103900.021.37767851
Produced by FastQC (version 0.11.5)