FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21128499
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA47100.031.72821650
GTCGCCG48900.030.99373245
GCGTCGT50300.030.44857812
CGCCGTA51800.028.64462547
GCCGTAT51950.028.56096848
TGGTCGC57750.025.4708843
AGCGTCG62000.025.14974211
TCGCCGT58300.024.91998546
AGATCGG65900.024.3438321
CCGTATC62050.023.86079849
GATCGGA68300.023.7909492
AGAGCGT66250.023.4844539
CATACGA15150.022.50321815
GGTCGCC69000.021.91712244
CGTCGTG72100.021.86760713
CGGTGGT71700.020.86978140
CGTGTAG74650.020.80608616
TCGGTGG74300.020.79366339
TCTCGGT74800.020.71680637
GTAGATC75900.020.49538832
Produced by FastQC (version 0.11.5)