FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859037

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859037
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22781564
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG29750.017.82301945
TATGCCG35800.015.09925748
GTTCCGT39950.013.78966135
CGTATGC38800.013.75458546
CTCGTAT40900.013.13254344
TATCTCG40300.012.9863241
CGTATCT40400.012.9542639
TCCGTAT41600.012.66334637
CCGGGAT45000.011.5894821
TCGCTCG31200.011.58765614
CCGTATC45150.011.51514938
TCTCGTA46750.011.48921943
CGCGTAT3305.1773553E-511.2083971
TTCCGTA47350.011.19827536
ACACGTC51300.010.8061113
CGCGAAT11700.010.7485661
AGTTCCG55550.010.53695934
CGCGATT8200.010.3746011
CGAAGAT74750.09.74793151
ATCTCGT56650.09.60295142
Produced by FastQC (version 0.11.5)