FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22781564
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG34250.013.80063214
CCGGGAT47400.013.1851311
CGACTAA19250.010.75300828
ACTCGTG46050.010.41347222
CGCAGAC73450.010.1072011
CGAAGAT73500.010.0533471
CCGGAAT39100.09.8906651
CGGGGAT49500.09.8355381
ACCCGAT46800.09.80411968
CGCTCGT47900.09.72382715
ACTTCGC52600.09.51032811
CCGGGTT59250.09.4991221
CCCGATA49950.09.32397569
CGTACTC60850.09.29870119
CGCGTAT4109.012317E-59.2638831
AACCCGA54450.09.18698367
CGCCAAT41800.09.1691891
TACTCGT53650.09.13130521
CTCGTAC50750.09.10977717
CGCGGAT23950.09.0827891
Produced by FastQC (version 0.11.5)