FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859041

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859041
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27002062
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG95200.036.569831
TCGTATG112800.033.06156544
TATGCCG116450.031.6100647
CGTATGC119300.031.17300845
CTCGTAT122050.030.81027243
TATCTCG122200.029.89738340
GATCGGA117450.029.674582
ACACGTC129850.029.6501914
ACCGATG129950.028.59168833
TCTCGTA136350.027.47787342
CCGATGT135650.026.9070634
ACGTCTG145250.026.7915716
CGATGTA138750.026.6042635
CACCGAT139900.026.45954732
CACGTCT146200.026.2163315
CACACGT156700.024.65788713
ATGCCGT152600.024.1444448
TCGGAAG155650.023.9420384
ATCGGAA146050.023.8872763
ATCTCGT159350.023.57676741
Produced by FastQC (version 0.11.5)