FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27002062
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA70400.051.30562650
GCGTCGT76300.047.51987512
GCCGTAT77900.046.68559348
CGCCGTA81350.044.62150647
GATCGGA86700.044.5263942
AGCGTCG91000.040.18450511
AGAGCGT92400.039.911639
AGATCGG96100.039.427221
CCGTATC93100.039.0184449
GTAGATC95000.037.991132
TGGTCGC99100.035.93565843
CGTCGTG104450.034.77938513
GTCGTGT103750.034.71547314
GTATCAT105300.034.4643851
GGTCGCC106750.033.843344
TCTCGGT108900.033.67500337
AAGAGCG114050.033.060338
ATCGGAA114600.032.9685483
TCGCCGT110600.032.4165746
GAGTGTA102500.032.33232528
Produced by FastQC (version 0.11.5)