FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27556538
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG60350.032.69670543
ACACGTC73550.030.57978613
TATGCCG64450.030.35485646
CGTATGC66150.029.88587844
GATCGGA68150.029.4173491
ACGTCTG81900.027.25107215
ATCGGAA78050.025.4606082
CTCGTAT77000.024.86435942
CACGTCT91200.024.7750914
TCTCGTA79100.023.76817541
ATCTCGT88150.021.21059240
ATGCCGT95900.020.97581147
GCACACG112400.020.37845811
CACACGT110600.020.36702312
TCGGAAG104500.019.2469863
GCCGTCT106350.018.81837749
CGTCTGA123000.018.565816
TTACACG38300.017.56438632
AATCTCG46950.017.56010439
CCGTCTT114500.017.53831550
Produced by FastQC (version 0.11.5)