FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31213532
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG33650.017.7357443
TATGCCG37450.015.75188546
TCGCTCG51800.014.78499614
CGTATGC42700.013.49201744
CGCAGAC79600.013.0730951
CGCTCGT59450.012.99852815
CTCGTAT48500.012.02079642
ACACGTC53950.011.3822113
CGAAGAT106100.010.9483551
ACTCGTG72600.010.83394222
GATCGGA52550.010.6577921
CTCGTAC73400.010.48093117
TTACACG36850.010.39139332
CGCCTCG77400.010.07292829
CGCGAAT12750.010.0326691
CCGGGAT57700.09.8862851
TAAGCGA18850.09.88258746
ACGTCTG62600.09.80942715
GCTCGTA80700.09.66120916
CTCGGTT31200.09.640281
Produced by FastQC (version 0.11.5)