FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859048

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859048
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31213532
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG50000.016.0078414
CATACGA15000.014.03051115
CGCTCGT59100.013.66039715
CGCAGAC80900.013.0886211
TTACACG33100.011.57543832
ACTCGTG70500.011.49616222
CGGCATA23000.011.401566512
CTCGTAC72800.011.04215617
TAAGCGA20350.011.01620846
CGCGAAC8550.010.891851
TATATCG18300.010.75305135
GCATACG25750.010.71833914
CGACGAT25100.010.7182751
GCTCGTA79350.010.65251116
CGATCCA42450.010.5591884
CGAAGAT102250.010.524351
CGCCAAT55150.010.4441961
TCGTGCG78600.010.40036724
CGCCTCG81500.010.03304129
TACTCGT80250.09.97056721
Produced by FastQC (version 0.11.5)