FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859049

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859049
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29429366
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC429370.14589848792529203TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG99250.032.6181343
TATGCCG103550.031.22983646
CGTATGC107400.030.24067944
GATCGGA110250.029.6428361
CTCGTAT113050.028.45073742
GTCACGA115850.028.06849929
ACACGTC117250.027.85044513
AGTCACG119600.027.12989228
ACGATCA120850.026.93616732
CACGATC124500.026.0621331
ACGTCTG125150.026.03647415
ATCGGAA125100.025.9619792
TCTCGTA129900.024.8410341
CACGTCT134850.024.2155314
ATCTCGT135050.024.02331740
CGATCAG136350.023.87411533
ATGCCGT140000.023.37391347
TCACGAT143000.022.78836430
TCGGAAG146050.022.3577043
GCCGTCT147700.021.9420749
Produced by FastQC (version 0.11.5)