FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30144501
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA38800.033.52145850
GCGTCGT51350.027.47869512
GCCGTAT48400.027.08650248
CGCCGTA54650.024.6200647
TGGTCGC60850.020.75050543
CCGTATC66550.020.11399749
GATCGGA77150.019.6775862
AGCGTCG72650.019.61239211
GTCGCCG70650.019.04410745
AGAGCGT76400.018.9657759
GTAGATC77550.017.66121132
TCGTGTA83700.017.30943915
TCGCCGT76750.016.90145746
ATCGGAA92100.016.7830663
GGTCGCC81000.016.69584844
CGTGTAG86600.016.61012816
GTCGTGT89200.016.12635214
GTATCAT85450.015.90728151
TCGGTGG88950.015.86385239
TCTCGGT88250.015.71599637
Produced by FastQC (version 0.11.5)