FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31619115
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG60050.028.82954243
TATGCCG65650.026.57758946
CGTATGC67500.025.7483944
CTCGTAT68250.025.21626942
GATCGGA78650.021.9816821
AATCTCG79050.021.34090239
ACACGTC88500.020.25386213
TCTCGTA84500.020.0449841
ATCGGAA88100.019.520092
ACGTCTG98200.018.35707715
ATGCCGT95200.018.25647547
ATCTCGT93400.018.17132640
CACGTCT104550.017.33978514
GCCGTCT111000.015.56588249
TCGGAAG120200.014.7541633
CCGTCTT117450.014.71104950
TGCCGTC119300.014.68249148
CGTCTGA129400.014.19379816
CACACGT129450.014.05698712
GCACACG143050.013.41009211
Produced by FastQC (version 0.11.5)