FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31862394
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG42350.020.41660333
TCGTATG42700.016.37405845
CGTATGC46750.015.17425146
TATGCCG45500.014.84155348
TTACACG33800.014.83502232
AGTCACG65400.013.66365228
GTCACGT71200.012.88705629
ACACGTC72600.012.63765213
GATCGGA61050.012.5759821
TAAGTCG32550.012.57335120
CTCGGTT41700.012.5198871
CTCGTAT58950.012.496840544
AATCTCG64850.012.25391641
GTTTCGG77100.012.06188934
CCCGTAT38300.012.027631
GTATCGA33500.012.0131054
ACGTCTG77500.011.83862615
CGTATCG25700.011.811573
CTCGGGT68100.011.5747041
CGGAATC75300.011.43017538
Produced by FastQC (version 0.11.5)