FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31862394
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA32750.017.69700650
TTACACG38050.015.59149132
GCCGTAT37300.015.44457848
CGTATCG26150.014.7799263
GCGTCGT41400.014.26629812
GTATCGA32650.014.0570784
GATCGGA49500.013.0362532
CCCGTAT37650.013.0150511
CGCCGTA48100.012.69334847
CTCGGGT74600.011.4719011
TAAGTCG31100.011.42349220
CGAAGAT120250.011.27794551
AGCGTCG53150.011.11307611
CCGTATC55200.010.99956249
CTGGAAT249950.010.72731
TCGGGTC65400.010.6055862
CTCGGAT81050.010.6015381
CTCGGTT43400.010.5750991
ATCGCGC32000.010.56427767
CGTTCGC27500.010.53609216
Produced by FastQC (version 0.11.5)