FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22168918
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA36500.035.16172850
CGCCGTA40600.032.03542747
GCGTCGT43000.031.77137612
GCCGTAT42200.030.49380348
CATACGA19900.028.60216915
GTCGCCG49750.026.48991445
GATCGGA57200.026.299342
CGGCATA24300.025.55498112
TGGTCGC51000.025.23166843
GCATACG26750.025.01773614
AGCGTCG54900.024.88479811
CCGTATC52850.024.74060849
TCGCCGT51000.024.48801446
ATCGGAA67700.023.239653
AGAGCGT61450.022.7939879
CGTCGTG61050.022.5460813
GGTCGCC59700.022.19035344
CGTGTAG64500.021.98099116
GTCGTGT63900.021.97169714
ATACGAG26550.021.04823516
Produced by FastQC (version 0.11.5)