FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34460628
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG46750.023.83599545
CGTATGC52700.021.27573246
TATGCCG52850.021.21537648
ACACGTC68800.017.70062413
CTCGTAT62450.017.6778744
GATCGGA62600.015.435061
CGTCCCG62450.014.74948135
ACGTCTG83600.014.69067115
CACGTCT89450.013.76861314
TCTCGTA81850.013.06670643
ACCCGTC71600.012.57550332
TTACGCG14950.012.23068854
CACACGT102700.011.92506712
GCGGTAT43500.011.7407821
GTCCCGA79100.011.73204536
TCCCGAT77500.011.57393237
ATCTCGT97350.011.19887142
ATGCCGT98850.011.09810849
AGATCGG68050.010.5984581
CCGATCT84000.010.51404839
Produced by FastQC (version 0.11.5)