FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859067

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859067
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28614625
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG20800.022.55638343
TATCGCG10800.017.24676722
TATGCCG29150.015.8577946
CGTATGC29600.015.38346144
CTCGTAT31650.014.71504342
CGCGTAT4651.0913936E-1113.4039121
AGTCACG39900.013.14179428
ATCGGTA28300.012.67598118
TTACGCG37100.012.0875754
CCAATCG31800.011.9315815
GCGGTAT54750.011.8901061
GATCGGA44400.011.854211
ACACGTC45100.011.77813613
CCGACAT52050.011.5089941
TAGACCG24000.011.49993558
ATCGAAG68100.011.3459916
TCGACCG51050.011.21740931
TAGCGAC37400.011.16080426
TACGCGA40500.011.15800755
GTTACGA19800.010.8019314
Produced by FastQC (version 0.11.5)