FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18654323
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA674650.3616587961943191TruSeq Adapter, Index 21 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA587840.3151226662045039TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG184000.048.562511
TCGTATG279550.043.28341745
CTCGTAT279800.041.955844
TCTCGTA283550.040.81499543
AATCTCG285500.040.66565741
CGTATGC304950.039.65165746
ATCTCGT291950.039.55903242
CGTTTCG340050.036.646533
TATGCCG333600.036.57193848
ACACGTC353850.035.41808313
AGTCACG348850.035.3816728
GTCACGT352700.035.08202429
GTTTCGG359250.035.00903734
ATGCCGT347150.034.89035849
GCCGTCT347350.034.84096551
GATCGGA364250.034.827261
ACGTCTG361350.034.74870315
CGGAATC340900.034.70602838
TTTCGGA360900.034.6421635
ACGTTTC360900.034.58575432
Produced by FastQC (version 0.11.5)