FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859070

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859070
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18654323
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG177850.049.1757161
CGAAACG35950.047.87627427
CATACGA33750.044.80971515
CGTATCA185900.044.78318450
CGCCGTA193450.043.8015147
GCCGTAT192350.043.4066748
GTCGCCG194500.043.3251245
TGGTCGC191850.043.30587843
CGGCGTG41200.043.1433412
GCGTCGT198800.042.56625712
TCGCCGT193100.042.50886546
CGGCATA41250.042.50420412
CCGTATC199900.041.75013449
GCATACG42100.041.68608514
TCTCGGT209500.041.5140337
CGGTGGT203200.041.0790140
AGCGTCG213300.040.27022611
GGTCGCC215350.039.55562644
TCGGTGG221050.039.5109439
CGTCGTG214350.039.4786113
Produced by FastQC (version 0.11.5)