FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32488663
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG42300.017.463363
GTATCGA36800.017.3523184
CCCGTAT42800.015.2430691
CGCGTAA18600.015.2179191
CAATCGG50750.014.6909984
AATCGGG54050.013.9214265
ATCGAAG67600.013.6327296
GTCGCTA21950.013.52326127
TCGATTG45550.013.48543220
CCGTATC67550.012.7241382
CGGTATT42900.012.7131792
TACTCGA49800.012.4686417
GACGCTA52350.012.2639364
CCGGTAT49350.012.1007791
GCGGTAT56150.012.0492681
ACTCGAT52500.011.96043318
CGCGCGT31000.011.9145171
CGGTATA23350.011.8265332
GCGGGTT40350.011.8056511
GCCGATT38300.011.7165281
Produced by FastQC (version 0.11.5)