FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31361410
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA89450.049.13706250
GCGTCGT94000.046.7346512
GCCGTAT95250.046.5180248
CGCCGTA98400.045.20404447
GATCGGA109850.043.241232
AGCGTCG112500.039.72272511
CCGTATC113950.038.93466649
AGAGCGT117400.038.2120939
AGATCGG121800.037.9126361
CATACGA34350.036.98445515
GTAGATC118950.036.40709732
TGGTCGC121500.036.27025243
CGTGTAG121650.036.0275416
TCGCCGT122950.035.50694746
GCATACG41350.034.97049314
CGTCGTG126500.034.9460113
ATCGGAA135300.034.8836253
GTCGTGT125800.034.59349414
CGGCATA43000.034.58847812
TCGTGTA126250.034.52406715
Produced by FastQC (version 0.11.5)