FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26003653
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA12000.010.49969343
CGTATCG23900.09.8118623
CGACCTA12800.09.5705114
CGACCGT13550.09.5570441
CTCGGTT34600.09.508521
CCGCAAT31600.09.303651
TCGGGTC49250.09.2386462
CGAAGAT80750.09.1887081
CGCGTAT5351.7446528E-69.1587341
CGTTCGC18150.09.062572516
CTCGGGT65050.09.0390581
CGATCGG9701.8189894E-129.0208054
GTATCGA28400.08.9966654
CCCGTAT33100.08.8820341
CGCGTAA3955.5402383E-48.860621
CCGGGAT56350.08.81973651
TTACACG25550.08.76668232
CTCGGAT60650.08.7137951
CAATCGG30700.08.6646754
CGCCGAT40150.08.631
Produced by FastQC (version 0.11.5)