FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17035461
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA19950.034.9088815
GCATACG24750.034.06996514
CGGCATA27050.032.70118712
CGTATCA52700.028.16910250
ATACGAG24800.027.3935816
CGCCGTA54950.027.07707247
GCCGTAT55800.026.54135748
GTCGCCG58100.025.25706745
CCGTATC60950.024.467249
TGGTCGC58550.023.78086543
GCGTCGT63900.023.57050112
AGATCGG67250.023.341941
TCGCCGT61500.023.3058446
GGTCGCC67400.021.67096544
AGCGTCG71100.021.22871811
AAGACGG56850.020.357918
AGACGGC56250.020.2158879
GATCGGA79100.020.0600192
TCTCGGT73600.019.84160637
CGTCGTG78300.019.54234513
Produced by FastQC (version 0.11.5)