FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859083

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859083
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30642086
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG33800.014.28939614
CGATAAC23250.012.32391551
CGGACAT46300.012.0958521
CGAAGAT96000.011.6674581
CGCTCGT41600.011.61013515
TAAGCGA19950.011.25216946
CGCAGAC70000.010.667391
CGTATCG24950.010.6386793
GTCGTTA21200.010.58871620
GTATCGA30450.010.4326714
GTACGAT32600.010.32886966
CCGCAAT31950.09.9192711
CGGAAAT60450.09.7672071
CAATCGG35500.09.7414994
CCGGGAT49550.09.7253911
CCGGCAT45300.09.6794561
ATAACGT27900.09.6579493
CGTGTCG36050.09.62343455
CGCCAAT49900.09.4831731
CGCGTCT17050.09.4211751
Produced by FastQC (version 0.11.5)