FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30642086
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA16250.013.58885315
TCGCTCG36850.012.92109214
CGAAGAT98400.011.0074271
CAATCGG34300.010.9612014
CGCTCGT44500.010.69979115
AATCGGG36900.010.1886675
GTATCGA33750.010.11782554
TACTCGA29400.010.092717
CGACCTA13400.010.0388874
TAAGCGA18550.09.6676346
CGTATCG27400.09.5674433
CGGCATA21650.09.56204912
CGGACAT48600.09.3689071
CGCAGAC74050.09.3631561
GACGCTA32150.09.333924
CGATAAC25300.09.271281
GTACGAT33200.09.24557125
ACTCGTG54000.09.06889622
CCGGCAT44800.08.9316421
CGACAAT48300.08.9271771
Produced by FastQC (version 0.11.5)