FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859085

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859085
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31447225
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG58300.036.50778243
CGTATGC64500.032.78457644
TATGCCG66900.031.76313646
CTCGTAT66850.030.65159842
GATCGGA77250.028.1051561
ACACGTC83750.026.36229913
TCTCGTA90450.022.76843541
CACGTCT101300.021.69316914
ACGTCTG107300.020.60873215
ATGCCGT107500.020.34452447
ATCGGAA108950.019.6028522
CACACGT115300.019.29794112
TCGGAAG113550.019.170793
ATCTCGT113850.018.11905740
GCCGTCT122200.017.24800549
AATCTCG122100.017.14909439
ACCAATC122600.017.13578236
GACCAAT129150.016.93844435
GCACACG139450.016.62344411
TGCCGTC137200.015.94057548
Produced by FastQC (version 0.11.5)