FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31447225
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA22350.026.85835315
GCATACG28800.025.63629314
CGGCATA33850.022.62699512
ATACGAG30850.018.55883416
CGTATCG30800.013.7819293
CCCGTAT32100.012.4712091
GCGGTAT42850.012.080811
GTATCGA31150.012.0753934
TTACGCG17500.011.62501554
CGCGTAA24500.010.9871261
GTCGCTA27800.010.92546227
CGATCCA43350.010.8263634
ATCGAAG65100.010.6547956
CGTTCGC22500.010.577044516
ATACGCG5251.3875251E-810.51134916
CCGACAT55400.010.2785011
AGACGGC94200.09.9988779
CGACCTA15100.09.8270754
TACGAGA53650.09.77221517
TACGCGA21850.09.62400355
Produced by FastQC (version 0.11.5)