FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31938994
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG12350.015.36730620
TTACGCG43300.013.45860454
CGTATCA25450.013.28505450
GCGGTAT85200.012.7578171
TACGCGA48200.012.16074755
GTATCGA30800.011.9876594
ATCGAAG73800.011.782576
TCGACCG53000.011.45473431
CGACCTA13450.011.2883824
CGACCGT53400.011.1104432
CCGACAT63750.010.8798081
ATCGGTA33350.010.86077118
TAAACGC82900.010.65246152
GTTACGC55750.010.64160753
CCAATCG35500.010.49383115
GCGTCGT32600.010.37038212
GGTATCC103400.010.14530853
TAACGCG14100.010.0294633
TAACGCC64000.09.97585829
CGACATC66800.09.8149792
Produced by FastQC (version 0.11.5)