FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14165653
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG46850.026.43629545
TATGCCG48750.025.9053648
CGTATGC50000.024.91267646
CTCGTAT50200.024.53458444
ACACGTC56100.023.18603113
TATCTCG53850.023.06396941
GTCACGA55750.023.01874429
GATCGGA58100.022.4895881
AGTCACG58250.022.2084828
CACGAGT55550.021.4869931
ACGTCTG62550.021.0690115
ATCGGAA62100.021.0556892
ACGAGTG57500.020.6383132
TCTCGTA59900.020.44634443
TCACGAG64350.020.31768230
CACGTCT67450.019.6936714
ATCTCGT63750.019.21168542
ATGCCGT65900.019.16367749
AGATCGG47650.018.3536931
GCCGTCT71950.017.9838951
Produced by FastQC (version 0.11.5)