FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27541534
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAT47150.014.3249751
GTATCGA26900.013.9240764
CGTATCG29400.013.5507873
ATCGAAG59200.012.1938296
CCCGTAT32900.011.3016271
CGCGTAA21550.011.2388391
GTCGCTA23600.010.96547827
CGGTATT33550.010.6566992
TTACGCG16500.010.52495154
CCGACAT48650.010.51765251
CGAGCGA41950.09.82160753
CCGCAAT26850.09.7826571
GCGGGTT33550.09.557531
CGCGGAT22600.09.5091681
TATCGAA38500.09.463445
TAACGCC28800.09.45852729
TTACACG28900.09.30804332
CCGGCAT47800.09.27739051
CCGTATC47150.09.2438782
TACGCGA19050.09.11614955
Produced by FastQC (version 0.11.5)