FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14726734
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAAC148010.10050429375583207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG26900.040.39502743
CGTATGC27700.038.97918344
TATGCCG29550.036.88907246
CTCGTAT30850.034.44016342
ACACGTC33650.033.00826613
GATCGGA34950.032.0376741
CACGTCT42250.026.2077814
ATGCCGT42100.025.64663747
CACACGT45800.024.7789212
TCGGAAG47900.023.3336183
TCTCGTA51750.021.33184641
GCCGTCT50600.021.20205949
GTATGCC52850.020.95213145
GTAATCT52200.020.81765437
TGCCGTC55750.019.73849148
TAATCTC55700.019.6952838
ATCGGTA19050.018.6506618
CCGACAT32400.018.189031
CGTCTGA61050.018.13724516
CTTGTAA65000.017.67350234
Produced by FastQC (version 0.11.5)