FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14726734
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCATAAC148440.10079627974539365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGTA18550.018.41077218
CCGACAT31650.017.8779931
ATCGAAG38550.017.5371636
CCAATCG19750.017.46841215
GCGCGTA5150.017.42321669
GCGGTAT64000.016.8738021
CGCTATG30850.016.22051629
GTATCGA11500.016.198244
CGGTATT28150.016.0529372
AAGCGAC30600.015.89457620
CGAAGGA34700.015.8054048
TCGCTAT30500.015.72775828
CCGGTAT29900.015.6933751
CGACATC35250.015.559642
TCGAAGG42950.015.4996457
CCATAAC80100.015.42455869
CGAGCGA79500.015.05910453
GAACGCT33700.014.84703735
GGTATCC67400.014.7402383
ACGCTTG33450.014.64852737
Produced by FastQC (version 0.11.5)