FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859097

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859097
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25306997
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT33100.026.85755334
TACGTAA38850.022.97258636
TCGTATG40200.022.63737545
CCGTACG41300.022.3724633
GTACGTA41700.021.40260135
ACCGTAC44500.021.15669632
CGTAATC43500.020.11468538
TATGCCG46500.019.72095548
ACGTAAT46700.019.560737
CGTATGC48200.019.09798246
CTCGTAT49700.018.16942644
GATCGGA53350.016.8648051
ACACGTC59900.016.71063813
AATCTCG55850.016.10533741
TCTCGTA57150.015.80085243
ACGTCTG67600.014.75663215
CACCGTA65500.014.58736831
ATCGGAA62950.014.2895282
CACGTCT77650.013.02643914
CGTTCGC18700.012.72788916
Produced by FastQC (version 0.11.5)