FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37958272
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA40250.013.5642550
CGTATCG38550.013.4372343
GTATCGA44150.012.5255294
GCCGTAT45800.011.99710348
TCGCGCG9550.011.7277478
CCCGTAT54100.011.70933151
TTACACG42000.010.91568832
TACCGTA30500.010.9017959
GCGTCGT55050.010.36397312
CGACCTA18400.010.2717784
ACCGTAT27100.010.07427710
GTCGCCG57550.09.91252945
CGACCGT22050.09.6821561
CTCGGAT92400.09.355691
ATCGCGC47800.09.29924767
CGAAGAT132400.08.9611241
TCGCTCG54600.08.8459322
TCGGGTC59250.08.8014312
CTCGGGT83550.08.7967781
CTCGGTT49400.08.7850761
Produced by FastQC (version 0.11.5)