FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859101

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859101
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33821339
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG60700.030.64020246
ACACGTC61400.029.8463113
CTCGTAT61650.029.01056342
GATCGGA64950.027.2388291
CGTATGC72750.026.05103944
TCGTATG75500.025.41463343
CACGTCT82300.022.34918614
TCTCGTA81500.021.86167541
ACTTAGG84700.021.84212532
ATGCCGT85250.021.67805947
CACTTAG87650.021.10699531
ACGTCTG90150.020.4406615
ATCTCGT90900.019.89906740
ATCGGAA95050.018.7895342
TTAGGCA100800.018.75638234
TCACTTA101750.018.24837130
CACACGT100750.018.22251112
TCGGAAG102250.017.426593
CTTAGGC106950.017.39357233
GCCGTCT110450.016.80324749
Produced by FastQC (version 0.11.5)