FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859102

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859102
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33821339
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT38250.017.140421
CGTATCG48900.013.5493573
TTACGCG13950.013.34707254
GTATCGA51200.012.6710784
CCGTATC54750.012.6688442
CGGAAAT72500.011.7559651
AATCGGG61800.011.3356255
CGATCCA49800.011.0177644
CGCGTAA21300.011.016121
GCGGAAT40850.010.89674951
GCGGGTT39800.010.4906311
TCGATTG40800.010.48752620
TTACACG35100.010.22067732
TACTCGA41900.010.2071517
CGAAATA41300.010.193772
CGAAGTA57300.09.99758722
CGAGCGA48650.09.99475353
GCGAAAT56700.09.98067951
GACGCTA45050.09.9580494
CGGGGTT84100.09.9293021
Produced by FastQC (version 0.11.5)