FastQCFastQC Report
Fri 3 May 2024
EGAF00004859103

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859103
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18667690
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTA643560.34474538627971646TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT628260.3365494070235793TruSeq Adapter, Index 19 (97% over 40bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG239870.12849474144899556Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)