FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859104

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859104
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18667690
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACGA78550.054.42495315
CGGCATA79800.053.2342212
GCATACG78500.052.5271814
ATACGAG81950.049.34823216
TACGAGA93800.041.9745317
CGTATCA133250.041.53293250
ACGAGAT94800.040.226318
ACGGCAT110250.039.08957711
AAGACGG114400.039.083718
AGACGGC115850.038.6578759
GATCGTA24250.037.81270622
GTATCAT148550.037.463951
AGATCGG162300.036.0387341
GGCATAC121050.035.5263913
GACGGCA121150.035.08857310
CGAGATC108150.034.85407619
GAAGACG138150.032.4395147
ATCATTA183700.031.4594453
GCGTCGT182150.031.08329612
TGGTCGC176400.031.0452743
Produced by FastQC (version 0.11.5)