FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78619701
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA207550.020.0477891
CGTATCA100400.019.00329249
GCGTCGT145950.017.08967411
GTAGATC194950.015.37696931
GCCGTAT129700.015.13647
AGCGTCG210950.015.07775310
AGAGCGT202800.015.0702948
CGGGTAG114050.014.94286115
ATCGGGA168450.014.0896752
GCCCGAT144050.014.03534947
ATCGGAA296250.013.9851862
AAGAGCG270600.013.8311127
TCGGAAG282500.013.6768353
GATCTCG295950.013.66143134
ATCATTA297900.013.57461352
GATCGGG181300.013.3211611
TTACGCG53650.012.60571554
CGCCGTA159700.012.37906846
GTATCGA82300.012.1558894
GGGGCGC392400.011.667171542
Produced by FastQC (version 0.11.5)