FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859107

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859107
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28035944
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG50250.033.08888243
CGTATGC56050.030.03414744
TATGCCG56500.029.79483246
ACACGTC63550.026.82093613
AGTCACG64600.026.0646228
GATCGGA65800.025.6386261
TATCTCG66400.024.57320639
CTCGTAT79100.022.1978142
CGCCAAT83400.021.26596833
CACGTCT80900.021.23945214
ACGTCTG85100.020.02899415
TCTCGTA84350.019.8346941
ATGCCGT87050.019.41765847
ATCGGAA90100.018.7241632
ATCTCGT89200.018.64021740
GTCACGC94150.018.1038729
CACACGT99900.017.33711212
TCGGAAG101200.017.1790623
GCCGTCT96800.017.1767649
TATCGCG19150.017.1128722
Produced by FastQC (version 0.11.5)