FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859108

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859108
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28035944
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA28000.029.574750
GCGTCGT31350.026.40157712
GCCGTAT36650.022.40621848
GATCGGA41250.021.3317662
CGCCGTA42800.019.6702647
AGAGCGT47900.018.0728959
TATCGCG18200.017.27455722
AGCGTCG50350.016.439111
AGATCGG52200.016.3281521
TGGTCGC52200.016.25159543
CGTATCG25600.015.2317233
GTAGATC58800.014.84157632
CCGTATC55700.014.80499649
GGTCGCC61700.014.3705244
GTCGTGT64450.014.17989214
TCGTGTA65400.013.97294215
CGTGTAG64500.013.73922316
CCCGTAT32150.013.41651251
ATCGGAA64200.013.276163
CGTCGTG64750.013.1024213
Produced by FastQC (version 0.11.5)