FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34993381
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG88750.035.5986843
CGTATGC94250.033.63113444
TATGCCG95150.033.3493746
CTCGTAT106800.030.06661442
ACACGTC111800.029.23565713
GATCGGA110500.028.6130051
TCTCGTA122100.026.10120441
CACGTCT134600.024.23004314
ATGCCGT131250.023.9927547
CACACGT145550.022.45648812
ATCTCGT141550.022.4416140
ACGTCTG147500.022.23880215
TCGGAAG148500.021.6519453
GCCGTCT152600.020.88459449
GTAATCT157600.020.13432737
GTATGCC165250.019.47371345
TAATCTC167100.019.3405438
ATCGGAA165200.019.2797812
TGCCGTC169100.018.82637448
CGTCTGA175800.018.77655616
Produced by FastQC (version 0.11.5)