FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34993381
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA46050.030.6427150
GCGTCGT53600.026.90293512
CGCCGTA59800.023.77005247
GATCGGA68400.022.1470812
GCCGTAT69500.020.5517648
GTAGATC76850.019.21081532
AGAGCGT75250.019.1639339
CCGTATC80200.018.28299949
AGATCGG82900.017.3155981
GTATCAT89000.016.31985351
CGTGTAG91000.016.14683316
GTCGTGT93050.015.83068314
TCGTGTA93100.015.78309515
AAGAGCG94550.015.3626188
CGTCGTG99700.015.25919313
GGTCGCC94400.015.16649944
TCTCGGT98300.015.08155237
TCGGAAG99950.014.9832164
AGCGTCG97300.014.67846311
TCGGTGG101750.014.50310139
Produced by FastQC (version 0.11.5)