FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859114

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859114
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28203349
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA14350.013.1711334
CGCGAAA12800.012.85149943
CGTTCGC21800.011.72014616
CGCGTTC22200.011.351315514
CGTATCG27550.011.1800943
CGACCGT12850.011.1676961
CGCGAGT10850.010.6455231
CCCGTAT33500.010.5525981
CCGCGTT25850.010.15470613
TCGGGTC51250.010.1076782
GTATCGA29550.09.9495424
CTCGGAT68850.09.7098441
CCGGGAT47000.09.60670951
TTCGCAC28050.09.48303518
CTCGGGT73350.09.44817351
CTCGTTT43850.09.4187931
CGCCGAC26700.09.3074321
CGCCAAC60300.09.0550351
CGACGAT20700.08.9616581
CGCGCGT9505.456968E-128.8424061
Produced by FastQC (version 0.11.5)