FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29959483
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG53700.022.93457645
TATGCCG65050.018.93267348
CGTATGC66400.018.70376446
CTCGTAT63500.018.36283544
GATCGGA64700.018.2786731
ACACGTC74600.016.87879413
ATCGGAA73200.015.9302252
AATCTCG72150.015.92215241
ACGTCTG81550.015.69413115
CACGTCT93050.014.01402114
TCTCGTA82050.013.91692443
ATCTCGT86150.013.53490742
ATGCCGT100950.012.06311149
CACACGT111950.011.64807912
CGTCTGA115250.011.28467816
TCGGAAG108750.011.2614713
TATCGCG8000.011.21306522
GCACACG124250.011.07804511
CCGGGAT63200.010.6694111
CGAAGAT92600.010.6054781
Produced by FastQC (version 0.11.5)